Using Biotinylated <i>myo</i>-Inositol Hexakisphosphate to Investigate Inositol Pyrophosphate–Protein Interactions with Surface-Based Biosensors

Inositol pyrophosphates (PP-InsPs) are highly phosphorylated molecules that have emerged as central nutrient messengers in eukaryotic organisms. They can bind to structurally diverse target proteins regulate biological functions, such protein–protein interactions. PP-InsPs strongly negatively charged and interact with basic surface patches proteins, making their quantitative biochemical analysis challenging. Here, we present the synthesis of biotinylated myo-inositol hexakisphosphates application plasmon resonance grating-coupled interferometry assays, enable rapid identification, validation, kinetic characterization InsP– PP-InsP–protein